Morphometric evaluation of the reparative regeneration of a wound closed with modified U-shaped staples with ciprofloxacine

The probability of complications of infectious and inflammatory etiology and the lack of effectiveness of surgical treatment when using U-shaped staples as a suture material determines the need for their improvement. One possible solution to this problem is the immobilization of antibacterial agents on a modified surface of the staple. In the framework of this study, the dynamics of reparative regeneration of an experimental “conditionally aseptic wound” in rats were investigated by stitching the muscle incision with U-shaped staples with calcium phosphate coating and ciprofloxacin incorporated in it. For the study, we used tissue fragments from wound sites adjacent to the staples. The samples were prepared from the microtome samples, which, after staining with hematoxylin and eosin, were morphometrically evaluated. The comparison group consisted of microscopic preparations of animals whose wound was closed with U-shaped staples without calcium phosphate coating and the drug. During the formation of the scar, manifestations of typical stages of the wound process - traumatic inflammation, development of granulation tissue, formation and the beginning of remodeling of the scar - were recorded. In the group of experimental animals, for stitching wounds which used U-shaped staples without calcium phosphate coating and ciprofloxacin, we observed a pronounced picture of exudative inflammation and slowing the maturation of granulation connective tissue, which ultimately led to the formation of a noticeable scar. The use of the staples with calcium phosphate coating and ciprofloxacin immobilized on it (0.045 % per a staple) reduced the severity of the inflammatory response. In the case of using implants with a ciprofloxacin content of 0.09 %, a lower rate of connective tissue regenerative reactions was noted per brace: the formation of relatively more pronounced scars was observed at the wound site.

titanium U-shaped staples, calcium phosphate coating, ciprofloxacin, morphometry

1. Алишихов, Ш.А. О методиках фиксации имплантатов в хирургии грыж (обзор литературы) / Ш.А. Алишихов, Н.Л. Матвеев, М.С. Наурбаев, Д.Ю. Богданов, А.А. Киреев // Эндоскопическая хирургия. - 2008. - № 6. - С. 60-63.

2. Bozhkova, S.A. Ortopedicheskaja implantat-associirovannaja infekcija: vedushhie vozbuditeli, lokal’naja rezistentnost’ i rekomendacii po antibakterial’noj terapii [Orthopedic implant-related infections: the pathogens, local resistance and recommendations for antibiotic therapy] / S.A. Bozhkova, R.M. Tihilov, M.V. Krasnova, A.N. Rukina // Travmatologija i ortopedija Rossii - Traumatology and orthopedics of Russia, 2013. - Vol. 4, № 70. - P. 5-15.

3. Gostev, V.V., Sidorenko, S.V. Bakterial’nyye bioplenki i infektsii [Bacterial biofilm formation and infections] / V.V. Gostev, S.V. Sidorenko // Zhurnal infektologii. - 2010. - Vol. 2, № 3. - P. 5-15.

4. Chernjavskij, V. K. Bakterial’nye biopljonki i infekcija (lekcija) [Bacterial biofilms and infection (lecture)] / V. K. Chernjavskij // Annals of Mechnicov Institute. - 2013. - № 1. - P. 86-90.

5. Golub, A.V. Bakterial’nye bioplenki - novaja cel’ terapii? [Bacterial biofilms - a new goal of therapy?] / A.V. Golub // Klinicheskaja mikrobiologija i antimikrobnaja himioterija - Clinical Microbiology and Antimicrobial Chemotherapy. - 2012. - Vol. 14, № 1 - P. 23-29.

6. Chebotar’, I.V. Antibiotikorezistentnost’ biopljonochnyh bakterij [Antibiotic resistance of bacterial biofilms] / I.V. Chebotar’, A.N. Majanskij, E.D. Konchakova, A.V. Lazareva, V.P. Chistjakova // Klinicheskaja mikrobiologija i antimikrobnaja himioterija - Clinical Microbiology and Antimicrobial Chemotherapy. - 2012. - Vol. 14, № 1. - P. 51-58.

7. Биокомпозиты на основе кальций-фосфатных покрытий, наноструктурных и ультрамелкозернистых биоинертных металлов, их биосовместимость и биодеградация / под ред. Н.З. Ляхов. Томск: Издательский дом Томского гос. ун-та, 2014. 595 с.

8. Попков, А.В. Биосовместимые имплантаты в травматологии и ортопедии (обзор литературы) / А.В. Попов // Гений ортопедии. - 2014. - № 3 - С.94-99.

9. Aves, E.P. Hydroxyapatite coating by solgel on Ti-6Al-4V alloy as drug carrier / E.P. Aves, G.F. Estevez, M.S. Sader, J.C. Sierra, J.C. Yurell, I.N. Bastos, G.D. Soares // Journal of Materials Science: Materials in Medicine. - 2009. - Vol. 20, № 2. - P. 543 - 547.

10. Шаркеев, Ю.П. Модифицирование поверхности титановых медицинских скобок для сшивающих аппаратов методом микродугового оксидирования / Ю.П. Шаркеев, В.В. Шейкин, М.Б. Седельникова, Е.В. Легостаева, Е.Г. Комарова, В.В. Ермаков, А.Н. Осипов, Е.А. Шелихова // Перспективные материалы. - 2015. - № 10. - С. 46-55.

11. Криволапов, Ю.А., Леенман, Е.Е. Морфологическая диагностика лимфом / Ю.А. Криволапов, Е.Е. Леенман. М.: «ИПК «Коста», 2006. 208 с.

12. Брайловская, Т.В., Федорина Т.А. Морфологические характеристики течения раневого процесса при экспериментальном моделировании резаных и рвано-ушибленных кожных ран / Т.В. Брайловская, Т.А. Федорина // Биомедицина. - 2009. - № 1. - С. - 68-74.